Research Tools

Body Weight Dose Calculator

Convert rodent study dosing protocols (mg/kg, mcg/kg) to absolute doses for any subject weight — or flip to calculate what mg/kg a given absolute dose represents. Pre-populated with published preclinical dosing data for 30+ research compounds.

Compound

Subject Weight

Recovery & Healing

Route

IP or SC

Frequency

Once daily

Dose range

2–10 mcg/kg

Reference

Sikiric et al. 2010; Sebecic et al. 1999

Effective across wide dose range (2–10 mcg/kg). IP and SC both well-validated in rodent models.

Absolute Dose

0.25 mcg

2.50e-4 mg

Subject Weight

25.0 g

0.0250 kg

Published Dose Range

Low

0.05 mcg

High

0.25 mcg

Your dose (0.25 mcg) is within range of published protocols.

Injection Volume by Reconstitution Concentration

Concentration	Volume (mL)	Syringe Units
0.1 mg/mL (100 mcg/mL)	0.0025	2.5 U
0.5 mg/mL (500 mcg/mL)	5.00e-4	0.5 U
1 mg/mL	2.50e-4	0.3 U
2 mg/mL	1.25e-4	0.1 U
5 mg/mL	5.00e-5	0.1 U

Syringe units based on 100 U/mL insulin syringe (1 U = 0.01 mL).

Published Rodent Dosing Reference

Recovery & Healing

Typical Dose	Range	Frequency	Route	Reference
10 mcg/kg	2–10 mcg/kg	Once daily	IP or SC	Sikiric et al. 2010; Sebecic et al. 1999
2 mg/kg	1–6 mg/kg	2× per week or daily	IP or SC	Philp et al. 2004; Bock-Marquette et al. 2004
1 mg/kg	0.5–3 mg/kg	Once daily	SC or topical	Pickart 1973; Leyden et al. 1994
500 mcg/kg	100–1000 mcg/kg	Once daily	SC or IP	Heffernan et al. 1999; Ng et al. 2000

GH Axis

Typical Dose	Range	Frequency	Route	Reference
100 mcg/kg	50–300 mcg/kg	Once to 3× daily	SC or IV	Johansen et al. 1999; Raun et al. 1998
100 mcg/kg	30–200 mcg/kg	Pulsatile, 2–3× daily	SC	Thorner et al. 1997; Walker et al. 1995
30 mcg/kg	10–100 mcg/kg	Once daily (nocturnal)	SC	Vittone et al. 1997; Sigalos & Pastuszak 2018
200 mcg/kg	100–300 mcg/kg	Once daily	SC	Stanley et al. 2009; Lo et al. 2010
10 mg/kg	5–30 mg/kg	Once daily (oral)	Oral gavage	Nass et al. 2008; Chapman et al. 1996
80 mcg/kg	40–160 mcg/kg	2–3× daily	SC or IV	Ghigo et al. 1994; Torsello et al. 2003

Metabolic

Typical Dose	Range	Frequency	Route	Reference
30 mcg/kg	3–100 mcg/kg	Once weekly	SC	O'Neil et al. 2018 (SCALE); Wilding et al. 2021 (STEP-1)
30 mcg/kg	3–100 mcg/kg	Once weekly	SC	Jastreboff et al. 2022 (SURMOUNT-1); Min et al. 2021
30 mcg/kg	3–100 mcg/kg	Once weekly	SC	Jastreboff et al. 2023 (NEJM Phase 2)
5 mg/kg	0.5–15 mg/kg	Once daily or 3× per week	IP	Lee et al. 2015 (Cell Metabolism); Young et al. 2021

Longevity

Typical Dose	Range	Frequency	Route	Reference
50 mcg/kg	10–200 mcg/kg	Once daily (10–20 day cycles)	IP or SC	Khavinson et al. 2003; Anisimov et al. 2003
500 mg/kg	100–1000 mg/kg	Once daily	IP or IV	Gomes et al. 2013 (Cell); Canto et al. 2012
3 mg/kg	0.1–10 mg/kg	Once daily (chronic) or acute IV (I/R)	SC (chronic) or IV (acute)	Szeto 2008 (Biochim Biophys Acta); Siegel et al. 2013
1 mg/kg	0.5–5 mg/kg	2× weekly	SC or IP	Goldstein et al. 1981; Li et al. 2017

Nootropics

Typical Dose	Range	Frequency	Route	Reference
300 mcg/kg	100–1000 mcg/kg	Once daily	IP or intranasal	Kozlovskaya et al. 2002; Semenova et al. 2010
100 mcg/kg	50–500 mcg/kg	Once daily	IP or intranasal	Dolotov et al. 2006; Agapova et al. 2007
30 mcg/kg	10–100 mcg/kg	Once daily (evening)	ICV or IP	Monnier et al. 1977; Graf & Kastin 1986
100 mcg/kg	50–200 mcg/kg	Once daily	IP, oral, or topical	Dalmasso et al. 2008; Mandal et al. 2009

Hormonal

Typical Dose	Range	Frequency	Route	Reference
100 mcg/kg	50–200 mcg/kg	Per experiment	SC or IP	Pfaus et al. 2004; Diamond et al. 2004
80 mcg/kg	40–200 mcg/kg	Per experiment	SC or ICV	Dorr et al. 1998; Erskine & Hanagan 1997
200 mcg/kg	50–1000 mcg/kg	Per experiment (pulsatile)	IV or IP	Dhillo et al. 2005 (JCEM); Abbara et al. 2017
1 mcg/kg	0.1–10 mcg/kg	Per experiment	IP or intranasal	Donaldson & Young 2008; Modi et al. 2014

Research Design Considerations

Allometric Scaling

mg/kg dosing does not directly translate across species due to differences in metabolic rate. Use allometric scaling (BSA or metabolic body weight) when extrapolating from mouse to rat or larger species.

Pair-Fed Controls

For metabolic peptides (GLP-1 analogs, MOTS-c), always include pair-fed controls to separate pharmacological effects from caloric restriction effects on body composition.

Route of Administration

IP and SC bioavailability differ. IP is faster but can irritate peritoneal tissue at high frequency. SC mimics clinical SC injection more closely and is preferred for chronic protocols.

Dose-Response Non-Linearity

Several peptides (AOD-9604, KPV, oxytocin) show inverted U-shaped dose-response curves. Always include at least 3 dose groups to characterize the full response curve.

Vehicle Controls

Match vehicle solvent, volume, and injection site between groups. BAC water at high volumes (>0.2 mL/mouse) can cause inflammation and confound results.

Sex Differences

GH axis peptides (sermorelin, tesamorelin, hexarelin) frequently show sex-specific IGF-1 responses. Include both sexes or justify single-sex studies explicitly in protocol.

Research Use Only. All dosing data is sourced from published preclinical literature and is provided for reference purposes only. Nexphoria does not provide veterinary, medical, or dosing advice. Dose values reflect published rodent study protocols and are not applicable to human use. Always consult published literature and institutional guidelines (IACUC) for approved study protocols. This calculator performs arithmetic only — verify all inputs and outputs independently.

Reconstitution Calculator

Calculate BAC water volume and dose volumes.

Cost Per Dose

Calculate research cost at your dosing protocol.

Dosing Frequency Planner

Build a weekly injection schedule.