Reconstitution Calculator

Reconstitution Protocol

1. 1

   Gather materials

   You need the lyophilized vial, bacteriostatic water (BAC water), a 1 mL or 3 mL syringe, and a clean work surface. Bacteriostatic water contains 0.9% benzyl alcohol as a preservative — use this for multi-dose vials to prevent bacterial contamination.

2. 2

   Wipe vial tops

   Clean both vial septa (the lyophilized peptide vial and the BAC water vial) with an alcohol swab. Let dry for 30 seconds before inserting a needle.

3. 3

   Draw BAC water

   Using the calculator above, draw the calculated volume of BAC water (10 mL) into your syringe. For small volumes (< 0.5 mL), a 1 mL insulin syringe is recommended for accuracy.

4. 4

   Inject slowly — aim at the vial wall

   Insert the needle into the lyophilized peptide vial. Direct the BAC water stream at the glass wall of the vial, not directly at the powder cake. This prevents foaming and maintains peptide integrity. Inject slowly.

5. 5

   Do not shake

   Gently swirl or roll the vial between your palms until the powder is fully dissolved. Shaking can denature peptides. The solution should be clear (colorless to faint yellow). Cloudiness may indicate contamination or incomplete dissolution — do not use.

6. 6

   Label and store

   Label your vial with the compound, concentration, date of reconstitution, and lot number. Store reconstituted peptides at 2–8°C (refrigerator). Most reconstituted peptides are stable for 4–8 weeks under cold storage. For longer storage, freeze at −20°C in aliquots.