FOR RESEARCH USE ONLY
NEXPHORIAResearch Grade Compounds
nexphoria.com | research@nexphoria.com
ISO-compliant quality management | 99%+ average purity
CERTIFICATE OF ANALYSIS
Sample / Reference Document
FOR RESEARCH USE ONLY — NOT FOR HUMAN CONSUMPTION
COMPOUND IDENTIFICATION
Product NameNAD+
Batch NumberNXP-202503-4140
CAS Number53-84-9
Lot NumberL2025-343
Molecular FormulaC₂₁H₂₇N₇O₁₄P₂
Manufacture Date2025-03-23
Molecular Weight663.43 g/mol
Expiry Date2027-03-23
AppearanceWhite to off-white crystalline powder
Test Date2025-04-23
HPLC PURITY ANALYSIS
Method
Reversed-Phase HPLC
Column
C18, 4.6×250mm, 5μm
Detection
UV @ 214nm / 280nm
Mobile Phase A
0.1% TFA in H₂O
Mobile Phase B
0.1% TFA in ACN
Gradient
5→95% B over 30 min
CHROMATOGRAM (representative)
| Peak | RT (min) | Area | Area % | Identity | Result |
|---|---|---|---|---|---|
| 1 | 5.0 | 3,045 | 0.33% | Unidentified impurity | — |
| 2 | 16.8 | 932,272 | 99.50% | Target compound | ✓ PASS |
| 3 | 22.1 | 1,640 | 0.17% | Related substance | — |
| Overall Purity | 99.50% | Specification: ≥99.5% | ✓ PASS | ||
MASS SPECTROMETRY (ESI-MS)
Theoretical MW (g/mol)663.43
Observed MW (g/mol)663.42
Ionization MethodESI-MS
MS ConfirmationPASS — [M+H]⁺ and [M+2H]²⁺ observed
ADDITIONAL QUALITY TESTS
| Test | Method | Specification | Result | Status |
|---|---|---|---|---|
| Appearance | Visual | White to off-white crystalline powder | White to off-white crystalline powder | ✓ PASS |
| Solubility | Visual (1 mg/mL) | Complete dissolution | Passes | ✓ PASS |
| Water Content | Karl Fischer | ≤8.0% | 0.2% | ✓ PASS |
| Bacterial Endotoxins | LAL (gel-clot) | <1.0 EU/mg | 0.07 EU/mg | ✓ PASS |
| Sterility | Membrane filtration | No growth (14 days) | Complies | ✓ PASS |
| pH (1 mg/mL, H₂O) | pH meter | 5.0–8.0 | 7.4 | ✓ PASS |
| Storage Compliance | Spec review | −20°C, anhydrous conditions | Complies | ✓ PASS |
CONCLUSION & AUTHORIZATION
✓ BATCH RELEASE — APPROVED
All specified tests conform to established acceptance criteria. Batch NXP-202503-4140 is released for research use. This certificate is issued under Nexphoria's quality management system.
Quality Control Analyst
Authorized Signatory — QC Department
Date: 2025-04-23
Quality Assurance Manager
Batch Release Authorization
Date: 2025-04-23
IMPORTANT NOTICE: This is a sample/representative Certificate of Analysis provided for reference purposes. Actual batch COAs are issued with each shipment and reflect the specific tested batch. This compound is sold exclusively for qualified in vitro and in vivo research. It is NOT approved by the FDA or any regulatory authority for human therapeutic use, veterinary use, or food/drug/cosmetic application. The purchaser is solely responsible for ensuring lawful acquisition and use in accordance with all applicable federal, state, and local regulations. Nexphoria makes no warranties regarding fitness for any particular purpose.
nexphoria.com | Batch: NXP-202503-4140 | Generated: 2026-06-02 | Document ID: COA-NAD-PLUS-SAMPLE
nexphoria.com | Batch: NXP-202503-4140 | Generated: 2026-06-02 | Document ID: COA-NAD-PLUS-SAMPLE